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four detector abberior stedycon sted unit  (abberior instruments)

 
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    Structured Review

    abberior instruments four detector abberior stedycon sted unit
    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x <t>STED</t> immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
    Four Detector Abberior Stedycon Sted Unit, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/four detector abberior stedycon sted unit/product/abberior instruments
    Average 90 stars, based on 1 article reviews
    four detector abberior stedycon sted unit - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth"

    Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

    Journal: bioRxiv

    doi: 10.1101/2025.07.09.663961

    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
    Figure Legend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Techniques Used: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay



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    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x <t>STED</t> immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
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    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x <t>STED</t> immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
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    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x <t>STED</t> immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
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    Image Search Results


    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Journal: bioRxiv

    Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

    doi: 10.1101/2025.07.09.663961

    Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

    Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay